DISTRIBUTION OF DEOXYNIVALENOL AND ZEARALENONE IN MILLED FRACTIONS OFWHEAT

Wheat samples collected from various locations in Kansas were milled a t 16% moisture content using a Buhler mill. Wheat bran, shorts, and fl our from samples invaded with Fusarium graminearum and several noninva ded samples were analyzed for deoxynivalenol and zearalenone using thi n-layer chromatography. The presence of deoxynivalenol and zearalenone on random samples was confirmed using gas chromatography. When presen t in milled fractions, the levels of deoxynivalenol and zearalenone we re generally highest in the bran and lowest in the flour. Ten of 27 br an samples (37%) and six of 27 shorts samples (22%) contained deoxyniv alenol levels that exceeded 1 ppm. Three of 27 samples (11%) yielded f lour with deoxynivalenol levels that exceeded 1 ppm. Zearalenone level s exceeded 1 ppm in five of 27 bran samples (19%) and four of 27 short s samples (15%). None of the flour fractions contained >1 ppm zearalen one.