EFFECT OF SOME CONSTITUENTS OF CHICKEN EGG-YOLK LIPOPROTEIN ON THE GROWTH AND IGM PRODUCTION OF HUMAN-HUMAN HYBRIDOMA CELLS AND OTHER HUMAN-DERIVED CELLS

Chicken egg yolk lipoprotein (YLP) was partially fractionated into som e constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 huma n-human hybridoma cell line cultured in serum-free medium. Among the f ractions, YP-1 and YP-2 fractions (LDL-rich fractions) were found to e nhance the growth and IgM secretion of HB4C5 cells. The promoting acti vity was found in the commercial LDL. The lipid fraction in YP-2 fract ion conjugated with 2-maltosyl-a-cyclodextrin was found to enhance the growth and IgM secretion of HB4C5 cells. Livetin-rich YP-3 and YP-4 f ractions had no significant promoting activity. Commercial gamma-livet in and phosphatidyl choline possessed no growth-promoting activity. Ph osphatidyl choline enhanced the IgM secretion of HB4C5 cells.