NEW CINEROMYCINS AND MUSACINS OBTAINED BY METABOLITE PATTERN-ANALYSISOF STREPTOMYCES-GRISEOVIRIDIS (FH-S-1832) .1. TAXONOMY, FERMENTATION,ISOLATION AND BIOLOGICAL-ACTIVITY

Chemical screening using thin layer chromatography and various stainin g reagents offers the opportunity to visualize a nearly complete pictu re of a microbial secondary metabolite pattern (metabolic finger-print ). This approach can be used advantageously for both, the detection of so-called ''talented'' strains, and for qualifying microbial strain c ollections, especially as a fundamental step of efficiently applied bi ological high-throughput assays. Based on their metabolic finger-print , microbial isolates can be classified in: (i) non-producing organisms , which gave no indication of the formation of secondary metabolites-u p to a defined detection limit, (ii) organisms of narrow productivity, which produce one or two secondary metabolites as main products with a restricted dependance to alteration of the culture conditions, and ( iii) talented organisms, which are able to synthesize an array of stru cturally different secondary metabolites. As an example, the talented strain, Streptomyces griseoviridis (FH-S 1832), was studied in detail. Investigations in its taxonomical characterization, fermentation, as well as the isolation and purification procedures leading to 14-member ed macrocyclic lactones of the cineromycin-type (cineromycin B and thr ee new congeners) and to the musacins A to F are reported. Musacin C e xhibits anthelminthic and weak antiviral activities.