AN IMPROVED METHOD FOR THE ROUTINE BIOPSY OF BOVINE MAMMARY TISSUE

Citation
Vc. Farr et al., AN IMPROVED METHOD FOR THE ROUTINE BIOPSY OF BOVINE MAMMARY TISSUE, Journal of dairy science, 79(4), 1996, pp. 543-549
Citations number
15
Categorie Soggetti
Agriculture Dairy & AnumalScience","Food Science & Tenology
Journal title
ISSN journal
00220302
Volume
79
Issue
4
Year of publication
1996
Pages
543 - 549
Database
ISI
SICI code
0022-0302(1996)79:4<543:AIMFTR>2.0.ZU;2-Z
Abstract
Eight primiparous cows in midlactation were used to determine a method for the mammary biopsy of standing cows in full lactation. Cows were mildly sedated; therefore, preoperative feed withdrawal was not necess ary. A core of secretory tissue (0.75 to 1 g) was extracted using a ro tating stainless steel cannula with a retractable blade at the cutting edge. Postoperative recovery was rapid, taking only 15 min per cow, a nd the method was reliable and efficient. The presence of secretory ti ssue was verified by histology and in situ hybridization with alpha(s1 )-casein and alpha-lactalbumin probes. The capsular end of the core co ntained more connective tissue, and the parenchyma showed heterogeneou s expression of alpha(s1)-casein and alpha-lactalbumin. Despite some p ostoperative bleeding, milk yield and composition in the biopsied glan d were affected only transiently. Yield recovered by 3.5 d after biops y, and composition recovered by 6.5 d after biopsy. Yield and composit ion of milk from the control glands were not affected by the procedure . Biopsy sites healed rapidly and without infection. No clinical masti tis was observed in any of the biopsied cows throughout the remainder of the lactation.