I. Cassarmalek et al., EXPRESSION OF ACETYLCHOLINESTERASE MOLECU LAR-FORMS DURING INVITRO DIFFERENTIATION OF RABBIT EMBRYONIC MUSCLE-CELLS, Comptes rendus de l'Academie des sciences. Serie 3, Sciences de la vie, 316(4), 1993, pp. 346-351
We investigated the myogenic properties of rabbit myoblasts in culture
, and examined particularly the expression of acetylcholinesterase (AC
hE). In the presence of horse serum, myoblasts proliferate quickly and
begin to fuse by day 3 of culture Differentiated myotubes presenting
some spontaneous contractions are clearly visible from days 6 to 9. Th
e cultures degenerate from day 10 onwards. The specific activity of AC
hE is low during the proliferation phase of myogenic cells, increases
sharply as myotubes differentiated, then decreases as cultures degener
ate. In 7-day-old cultures, globular forms are largely predominant and
asymmetric forms, detected as A12, represent only 1% of the total act
ivity.