IDENTIFICATION AND CHARACTERIZATION OF BPH2, A NOVEL HISTONE H1 HOMOLOG IN BORDETELLA-PERTUSSIS

Authors
Citation
S. Goyard, IDENTIFICATION AND CHARACTERIZATION OF BPH2, A NOVEL HISTONE H1 HOMOLOG IN BORDETELLA-PERTUSSIS, Journal of bacteriology, 178(11), 1996, pp. 3066-3071
Citations number
38
Categorie Soggetti
Microbiology
Journal title
ISSN journal
00219193
Volume
178
Issue
11
Year of publication
1996
Pages
3066 - 3071
Database
ISI
SICI code
0021-9193(1996)178:11<3066:IACOBA>2.0.ZU;2-2
Abstract
A basic protein, BpH2, with an apparent molecular mass of 18 kDa was p urified from Bordetella pertussis, and the corresponding gene, bph2, w as cloned. Sequence analysis revealed some homology to the H1 class of eukaryotic histones and to AlgP protein of Pseudomonas aeruginosa. Bp H2 binds both single- and double-stranded DNA in a nonspecific manner, Deletion of the corresponding gene in B. pertussis generated a BpH2 n ull mutant with an altered growth rate in which the expression of two virulence factors, adenylate cyclase-hemolysin (CyaA) and filamentous hemagglutinin (FhaB), was reduced. It is suggested that BpH2 may exhib it specific regulatory functions through its interaction with chromoso mal DNA.