Jm. Gomezgomez et al., CYCLIC-AMP RECEPTOR PROTEIN POSITIVELY CONTROLS GYRA TRANSCRIPTION AND ALTERS DNA TOPOLOGY AFTER NUTRITIONAL UPSHIFT IN ESCHERICHIA-COLI, Journal of bacteriology, 178(11), 1996, pp. 3331-3334
The expression of a transcriptional gyrA-lacZ gene fusion throughout t
he Escherichia coli growth cycle and the effect that mutation Delta cr
p39 had on this expression were studied. The data obtained indicate th
at the expression of gyrA is growth phase dependent and under the posi
tive control of the cyclic AMP receptor protein (CRP). Complementation
analysis of gyrA-lacZ expression with wild-type CRP or variant CRP(pc
) (with a T-to-A mutation at position 158) in a CRP-deficient backgrou
nd suggests that this CRP action is mediated by a class I or class II
CRP-dependent promoter(s). Our results also indicate that CRP may be i
nvolved in the modulation of DNA topology in the transition from the l
ag period to the exponential phase of growth.