ELIMINATION OF THE EFFECTS OF STRAY LIGHT IN MEASUREMENTS BY TOTAL INTERNAL-REFLECTION AQUEOUS FLUORESCENCE (TIRAF)

Authors
GINGELL D HEAVENS O
Citation
D. Gingell et O. Heavens, ELIMINATION OF THE EFFECTS OF STRAY LIGHT IN MEASUREMENTS BY TOTAL INTERNAL-REFLECTION AQUEOUS FLUORESCENCE (TIRAF), Journal of Microscopy, 182, 1996, pp. 141-148
Citations number
4
Categorie Soggetti
Microscopy
Journal title
Journal of Microscopy → ACNP
ISSN journal
00222720
Volume
182
Year of publication
1996
Part
2
Pages
141 - 148
Database
ISI
SICI code
0022-2720(1996)182:<141:EOTEOS>2.0.ZU;2-I
Abstract
Total internal reflection aqueous fluorescence has been shown to be ca pable of achieving spatial resolution in surface contours of about 1 n m. When used with highly structured objects, errors in measurements ca n arise from light scattered either by the object or within the body o f the microscope. We describe how these errors can be eliminated when studying surface contours of human platelets.