DETERMINATION OF ACETYLCHOLINE AND CHOLINE IN RAT-BRAIN TISSUE BY FIAWITH IMMOBILIZED ENZYMES AND CHEMILUMINESCENCE DETECTION

A simple, rapid. economical anti sensitive method for the determinatio n of acetylcholine and choline in neuronal tissue using a FIA system w ith immobilized enzyme and chemiluminescence detection is presented. T he analytes were injected into a continuous stream of a simple medium flowing through a sequence of enzyme reactors containing acetylcholine sterase and choline oxidase. The generated hydrogen peroxide was conti nuously monitored by a homemade chemiluminescence detector. The linear determination ranges were from 1 pmol to 1 nmol of acetylcholine and 1 pmol to 10 nmol of choline. The limits of sensitivity uf the procedu re were 600 fmol of acetylcholine and 500 fmol of choline. (C) 1996 Ac ademic Press, Inc.