As. Kochevenko et al., PROTOPLAST CULTURE AND SOMACLONAL VARIABILITY OF SPECIES OF SERIES JUGLANDIFOLIA, Plant cell, tissue and organ culture, 44(2), 1996, pp. 103-110
Mesophyll protoplasts of species of series Juglandifolia (Solanum rick
ii, S. lycopersicoides, S. ochranthum and S. juglandifolium) were isol
ated and cultured in liquid nutrient media TM-2 or KM8P. The cell colo
nies formed were transferred onto agar-solidified media TM-3 or GM, an
d 10 to 15 days later onto TM-4, PRM, MS3ZG, KK or C regeneration medi
a. Formation of the shoots for S. rickii and S, lycopersicoides was ob
served after 30 to 35 days on regeneration medium. The regenerated sho
ots were rooted on hormone-free MS medium. Morphological and cytogenet
ic analyses have shown that somaclonal variants might arise in the cou
rse of plant regeneration from protoplasts of these species.