PURIFICATION AND CHARACTERIZATION OF A LECTIN FROM XANTHOMONAS-CAMPESTRIS NCIM-5028

Authors
JOSHI B KHIRE JM SIVARAMAN H KHAN MI
Citation
B. Joshi et al., PURIFICATION AND CHARACTERIZATION OF A LECTIN FROM XANTHOMONAS-CAMPESTRIS NCIM-5028, Canadian journal of microbiology, 42(6), 1996, pp. 609-612
Citations number
20
Categorie Soggetti
Microbiology,Immunology,"Biothechnology & Applied Migrobiology",Biology
Journal title
Canadian journal of microbiology → ACNP
ISSN journal
00084166
Volume
42
Issue
6
Year of publication
1996
Pages
609 - 612
Database
ISI
SICI code
0008-4166(1996)42:6<609:PACOAL>2.0.ZU;2-Z
Abstract
A lectin was isolated from culture filtrates of Xanthomonas campestris NCIM 5028, by a simple procedure of hydrophobic chromatography on phe nyl-Sepharose after ammonium sulphate precipitation. The lectin was a heterodimer, with subunit molecular masses of 30 000 and 28 000. Gel f iltration on S-300 column, calibrated with markers, showed its molecul ar mass to be approximately 70 000. Its isoelectric point was 7.2. The agglutination of the rabbit erythrocytes by the lectin was inhibited by fetuin glycopeptides and host plant (Brassica oleracea) extracts.