DISPARATE TISSUE-SPECIFIC EXPRESSION OF MEMBERS OF THE TISSUE KALLIKREIN MULTIGENE FAMILY OF THE RAT

To understand the regulatory diversity of the rat Family of linked kal likrein genes, we have assayed the expression of family members in 20 major organs. Reverse transcription-polymerase chain reaction analysis using primers and hybridization probes specific for each of the 10 ex pressed kallikrein genes showed that no two family members share the s ame organ-specific pattern of expression. The only common site of expr ession for all 10 known active genes is the submandibular gland. The p resence of the mRNA for at least one family member is detected in 19 o f these 20 organs (liver excepted), from as few as three organs to as many as 18 for individual family members. For individual genes there c an be more than a 10(5)-fold variation in mRNA levels among organs, fr om a limit of detection of slightly less than 1 mRNA molecule/10 cells to more than 10,000 mRNA molecules/cell. Despite high sequence conser vation and close linkage, the members of this family are expressed in very different and complex patterns. A gradient of diversity of expres sion corresponds to the order of the genes within the kallikrein famil y locus.