DESIGN AND PROPERTIES OF NEW F-19 NMR CA2- MODULATION OF THE AFFINITIES OF BAPTA DERIVATIVES VIA ALKYLATION( INDICATORS )

The effects of alkyl substitution in the aminodiacetic groups of ,2-bi s(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) have been e xamined systematically with the aim of increasing the affinity for Ca2 + of novel F-19 NM R and fluorescent Ca2+ indicators based on the BAPT A structure into the range required to measure the cytosolic free Ca2 concentration ([Ca2+]i). Single methylation on the beta-carbon of eac h aminodiacetic acid group of BAPTA derivatives gave increases in the association constant for Ca2+ [K(Ca2+)] of up to 22-fold, depending on the structure of the parent BAPTA derivative. This was shown to be th e most effective alkylation for BAPTA derivatives to raise K(Ca2+) wit h a minimal increase in pK(a), and no loss of selectivity for Ca2+, so that the insensitivity of Ca2+ binding to pH and [Mg2+] over the phys iological ranges was maintained. (1) Single methylation on the beta-ca rbon of each aminodiacetic acid group of BAPTA derivatives symmetrical ly substituted with single fluorines at the 4-position (4FBAPTA) or at the 5-position (5FBAPTA) raised log K(Ca2+) from 5.61 to 6.81 and 6.1 2 to 7.47, respectively. The corresponding pK(av) values (average of t he two highest pK(a) values in the molecule) were increased from 4.50 to 6.02 and 5.85 to 6.59. The F-19 NMR spectroscopic properties of the methylated derivatives were unchanged compared with the parent compou nds except for slower exchange rates. These new methylated derivatives of 4FBAPTA and 5FBAPTA therefore extend the range of K(Ca2+) availabl e for F-19 NMR indicators of [Ca2+]i. (2) Single methylation on the be ta-carbon of each aminodiacetic acid group of BAPTA derivatives symmet rically substituted at the 5-position on the aromatic rings with trifl uoromethyl groups increased log K(Ca2+) from 4.83 to 6.14 and the pK(a v) from 5.39 to 5.90, respectively. There is a Ca2+-induced chemical s hift of the F-19 NMR resonance of 0.81 ppm upfield with slow exchange at 376 MHz and 30-degrees-C. This compound was selected as a prototype for F-19 NMR indicators with enhanced sensitivity through the incorpo ration of two trifluoromethyl groups to replace the two single fluorin e substituents in the current F-19 NMR indicators.