METABOLISM OF N-PHENYLLINOLEAMIDE BY RAT-LIVER

N-Phenyllinoleamide (NPLA), the anilide of linoleic acid, has been ass ociated with the epidemiology of toxic oil syndrome, but its contribut ion to the illness is still undetermined. Because it has been suggeste d that fatty acid anilides were absorbed via the hepatic portal vein, this study has been aimed at determining the hepatic metabolism of NPL A by rat liver. For this purpose, isolated liver was perfused with NPL A (0.1 mM) spiked with either aniline- or fatty acid-labelled NPLA. Ga s chromatographic-mass spectrometric analysis of the peaks appearing i n the radiochromatographic metabolic profiles shows that metabolism of NPLA in the liver results in formation of aniline and linoleic acid, both biologically active metabolites whose expected direct effects wer e not observed in patients suffering toxic oil syndrome.