DETERMINATION OF A NEW ANTIFILARIAL DRUG, UMF-058, AND MEBENDAZOLE INWHOLE-BLOOD BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A rapid and selective high-performance liquid chromatographic assay fo r simultaneous quantitative determination of a new antifilarial drug ( UMF-058, 1) and mebendazole (MBZ) is described. After a simple extract ion from whole blood, both compounds were analysed using a C18 Nova Pa k reversed-phase column and a mobile phase of methanol-0.05 M ammonium dihydrogenphosphate (50:50, v/v) adjusted to pH 4.0, with ultraviolet detection at 291 nm. The average recoveries of I and MBZ over a conce ntration range of 25-250 ng/ml were 92.0 +/- 7.7 and 84.4 +/- 4.4%, re spectively. The minimum detectable concentrations in whole blood for I and MBZ were 7 and 6 ng/ml, respectively. This method was found to be suitable for pharmacokinetic studies.