M. Nogawa et al., CLONING AND CHARACTERIZATION OF MYCOVIRUS DOUBLE-STRANDED-RNA FROM THE PLANT-PATHOGENIC FUNGUS, FUSARIUM-SOLANI F-SP ROBINIAE, Bioscience, biotechnology, and biochemistry, 60(5), 1996, pp. 784-788
A mycovirus (named FusoV) from the phytopathogenic fungus, Fusarium so
lani f. sp. robiniae SUF704, has two kinds of double-stranded (ds) RNA
genomes, designated M1 and M2. The cDNAs were constructed from FusoV
genomic dsRNAs, The sequences of M1 and M2 cDNAs comprised 1645 and 14
45bp, respectively, Sequence analysis showed that each dsRNA had a sin
gle long open reading frame (ORF) on only one of the strands, M1 ORF e
ncodes a 519-amino acid residue polypeptide with a predicted molecular
mass of 60 kDa, RNA-dependent RNA polymerase-conserved motifs were id
entified in the predicted amino acid sequence, and the polymerase synt
hesized dsRNA in vitro, The M2 ORF encodes a polypeptide of 413 amino
acid residues with a predicted molecular mass of 44 kDa, The predicted
amino acid sequence contained the sequence corresponding to those fou
nd in the purified 44-kDa capsid protein of FusoV.