REFINEMENT OF A THYROID CULTURED-CELL SYSTEM USED IN CLINICALLY RELEVANT BIOASSAYS

Thyroid cultured cells are now used worldwide in clinical bioassays of TSH and of thyroid autoantibodies. Having originally developed the th yroid cell cultures (Ambesi-Impiombato et al. 1980) from rat glands in our laboratory, we now aim to improve the system, moving in two direc tions: a) TSH-independent mutants have been produced and characterized , which can be used in clinical bioassays without ''starvation'' from the hormone. b) Human cultures have been attempted using our experienc e with rat cells, as well as innovative strategies. Preliminary result s now indicate that human normal differentiated cells may be available for clinical studies in vitro, when species-specific differences may be critical.