GAMMA-ENOLASE EXPRESSION AS EARLY MARKER OF NEURONAL DIFFERENTIATION OF MURINE NEUROBLASTOMA-CELLS N-115

Authors
CERVELLO M GIALLONGO A DAMELIO L SCIARRINO S MATRANGA V
Citation
M. Cervello et al., GAMMA-ENOLASE EXPRESSION AS EARLY MARKER OF NEURONAL DIFFERENTIATION OF MURINE NEUROBLASTOMA-CELLS N-115, Cytotechnology, 11, 1993, pp. 167-169
Citations number
6
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Cytotechnology → ACNP
ISSN journal
09209069
Volume
11
Year of publication
1993
Supplement
1
Pages
167 - 169
Database
ISI
SICI code
0920-9069(1993)11:<167:GEAEMO>2.0.ZU;2-S
Abstract
In this study we determined the levels of gamma enolase mRNA in mouse neuroblastoma cell line N-115 at early period of induction of differen tiation by serum withdrawal. The expression of gamma enolase was exami ned by Northern blot analysis of total RNA extracted from cells induce d for different lengths of time. We found a 3-fold increase in the lev el of gamma enolase mRNA after 24 hours of induction of differentiatio n and higher levels were detected in cells induced for longer time, re aching a 10-fold increase after four days.