PATHOGENIC ROLE OF ANTIENDOTHELIAL CELL ANTIBODIES IN VASCULITIS - ANIDIOTYPIC EXPERIMENTAL-MODEL

Citation
M. Damianovich et al., PATHOGENIC ROLE OF ANTIENDOTHELIAL CELL ANTIBODIES IN VASCULITIS - ANIDIOTYPIC EXPERIMENTAL-MODEL, The Journal of immunology, 156(12), 1996, pp. 4946-4951
Citations number
40
Categorie Soggetti
Immunology
Journal title
The Journal of immunology
ISSN journal
00221767 → ACNP
Volume
156
Issue
12
Year of publication
1996
Pages
4946 - 4951
Database
ISI
SICI code
0022-1767(1996)156:12<4946:PROACA>2.0.ZU;2-Q
Abstract
Idiotypic manipulation of naive mice has previously been used for indu ction of systemic autoimmune diseases (e.g., antiphospholipid syndrome , systemic lupus erythematosus, and Wegener's granulomatosis). The aim of this study focused on the utilization of this technique to induce the production of anti-endothelial cells Abs (AECA) and autoimmune vas culitis in a murine model, AECA were derived from a Wegener's granulom atosis patient's plasma. IgG was purified by absorption on a proteinas e-3 affinity column resulting in the depletion of anti-neutrophil cyto plasmic Ab activity. The absorbed IgG fraction displayed a high titer of AECA as evidenced by a cyto-ELISA against unfixed human umbilical v ein endothelial cells, BALB/c mice were actively immunized with the pu rified AECA. Three months after a boost injection with the human AECA, mice developed endogenous AECA (Ab3), but not Abs to proteinase-3, ca rdiolipin, or DNA. Histologic examination of lungs and kidneys reveale d both lymphoid cell infiltration surrounding arterioles and venules a s well as deposition of Igs at the outer part of blood vessel walls. T his experimental animal model of vasculitis, a product of our method o f idiotypic manipulation, provides the first direct proof for the path ogenicity of AECA.