CHARACTERIZATION OF NATURAL PEPTIDES FROM BOVINE TISSUE USING CAPILLARY ELECTROPHORESIS, HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, MATRIX-ASSISTED LASER-DESORPTION IONIZATION, AND EDMAN DEGRADATION

Authors
WELLER A STOEVA S LEHMANN R GRUBLER G PAULUS G VOELTER W
Citation
A. Weller et al., CHARACTERIZATION OF NATURAL PEPTIDES FROM BOVINE TISSUE USING CAPILLARY ELECTROPHORESIS, HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, MATRIX-ASSISTED LASER-DESORPTION IONIZATION, AND EDMAN DEGRADATION, Electrophoresis, 17(3), 1996, pp. 518-522
Citations number
11
Categorie Soggetti
Biochemical Research Methods
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
17
Issue
3
Year of publication
1996
Pages
518 - 522
Database
ISI
SICI code
0173-0835(1996)17:3<518:CONPFB>2.0.ZU;2-6
Abstract
A combination of high performance liquid chromatography (HPLC) and cap illary electrophoresis (CE) spiking, Edman degradation, amino acid ana lysis and matrix-assisted laser desorption ionization mass spectrometr y (MALDI-MS) was applied for the analysis of the primary structure of beta-thymosins. CE has been used to resolve peaks which coelute on HPL C, as well as to help establish the identity of tryptic fragments in t he peptide mapping experiments of the two highly homologous beta-thymo sins.