2-DIMENSIONAL GEL-ELECTROPHORESIS OF ESCHERICHIA-COLI HOMOGENATES - THE ESCHERICHIA-COLI SWISS-2DPAGE DATABASE

Numerous Escherichia coli proteins have already been characterized by two-dimensional gel electrophoresis (2-D PAGE), using carrier ampholyt es in the first dimension (VanBogelen, R. A., Sankar, P., Clark, R. L. , Began, J. A. and Neidhardt, F. C., Electrophoresis 1992, 13, 1014-10 54). We present here a reference protein map of E. coli obtained with immobilized pH gradients (IPG) and available in a SWISS-2DPAGE format. Out of the protein spots identified in the E. coli gene protein datab ase by Neidhardt's group, 153 have been identified on the E.coli SWISS -2DPAGE database map by gel comparison and most of them were confirmed either by the analysis of amino acid composition (AAC) and/or N-termi nal microsequencing. Additionally, five as yet unsequenced proteins we re found. The E. coli SWISS-2DPAGE database is part of the ExPASy mole cular biology server accessible through the Word Wide Web network.