2-DIMENSIONAL ELECTROPHORETIC PATTERNS OF ACUTE-PHASE HUMAN SERUM-PROTEINS IN THE COURSE OF BACTERIAL AND VIRAL DISEASES

Acute-phase serum proteins were analyzed by two-dimensional electropho resis with isoelectric focusing in 3-10 immobilized pH gradients. Most spots were identified by reference to the plasma map in the SWISS-2DP AGE database. Serum amyloid A protein spots were identified by immunob lotting with specific antiserum and by matching determined with predic ted values of electrophoretic parameters. Changes in the concentration s of al-antitrypsin, leucine-rich glycoprotein, haptoglobin, serum ret inol-binding protein and transthyretin were quantitated by densitometr y of silver-stained gels. Electrophoretic patterns from 18 patients wi th bacterial diseases and 16 patients with viral diseases were compare d. The incidence of serum amyloid A protein spots was 18/18 in bacteri al diseases and 6/16 in viral diseases. As for the other reactants stu died, variations were simultaneous in bacterial disease and tended to be staggered in viral diseases.