P. Serror et Al. Sonenshein, INTERACTION OF CODY, A NOVEL BACILLUS-SUBTILIS DNA-BINDING PROTEIN, WITH THE DPP PROMOTER REGION, Molecular microbiology, 20(4), 1996, pp. 843-852
The product of the codY gene is required for nutritional repression of
the Bacillus subtilis dipeptide permease operon (dpp), an operon expr
essed at early stationary phase in nutrient-rich medium. Though unrela
ted to any known DNA-binding protein, CodY was shown to bind specifica
lly to the dpp promoter region. DNase I footprinting experiments revea
led that the CodY-protected region encompasses the dpp transcription s
tart site and overlaps with the region protected by another regulatory
protein, AbrB. CodY and AbrB were found to compete, in vitro, for bin
ding to the dpp promoter region. Binding of CodY was altered in mutant
s defective in nutritional regulation.