THEORETICAL PERMUTATION GEL-ELECTROPHORETIC ANALYSIS OF A CURVED DNA FRAGMENT LOCATED IN CIRCULAR PERMUTATION

Authors
NAIR TM MADHUSUDAN K NAGARAJA V KULKARNI BD MAJUMDAR HK SINGH R
Citation
Tm. Nair et al., THEORETICAL PERMUTATION GEL-ELECTROPHORETIC ANALYSIS OF A CURVED DNA FRAGMENT LOCATED IN CIRCULAR PERMUTATION, Electrophoresis, 17(4), 1996, pp. 633-641
Citations number
32
Categorie Soggetti
Biochemical Research Methods
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
17
Issue
4
Year of publication
1996
Pages
633 - 641
Database
ISI
SICI code
0173-0835(1996)17:4<633:TPGAOA>2.0.ZU;2-T
Abstract
Using the theoretical model for DNA curvature, we analyzed a set of fr agments with a curved insert located in circular permutation. The theo retical permutation analysis of each of the cyclically located fragmen ts reveals the presence of a shifting molecular bend locus. The deline ation of the molecular bend locus associated with the fragments obtain ed by a second permutation helps in providing an explanation for the d ifferential mobility behavior of the fragments.