Gj. Stuart et al., PATCH-CLAMP RECORDINGS FROM THE SOMA AND DENDRITES OF NEURONS IN BRAIN-SLICES USING INFRARED VIDEO MICROSCOPY, Pflugers Archiv, 423(5-6), 1993, pp. 511-518
A description is given of the implementation of infrared differential
interference contrast (IR-DIC) video microscopy to an upright compound
microscope. Using the improved resolution offered by IR-DIC a procedu
re is described for making patch-pipette recordings from visually iden
tified neuronal somata and dendrites in brain slices. As an example of
the application of this technique to electrophysiological recordings
from small neuronal processes in brain slices we describe whole-cell c
urrent-clamp and cell-attached and excised patch-clamp recordings from
the apical dendrites of layer V pyramidal neurons in slices of rat ne
ocortex.