KINETICS AND THERMODYNAMICS STUDIES ON THE INTERACTION OF D-AMINO-ACID OXIDASE AND SODIUM N-DODECYL SULFATE IN THE PRESENCE AND ABSENCE OF FLAVIN ADENINE-DINUCLEOTIDE

The kinetics and thermodynamics of denaturation of D-amino acid oxidas e by sodium n-dodecyl sulphate (SDS) was studied in the presence and a bsence of additional amounts of flavin adenine dinucleotide (FAD) at v arious temperatures (310-325 K) in 0.02 M sodium pyrophosphate at pH = 8.3. The activity measurement data was gathered with thermodynamic da ta for further interpertation. The corresponding data shows higher sta bility for DAO against temperatures and potent denaturants like SDS.