A FLUOROMETRIC ASSAY OF CERAMIDE GLYCANASE WITH 4-METHYLUMBELLIFERYL BETA-D-LACTOSIDE DERIVATIVES

Authors
WANG LX PAVLOVA NV LI SC LI YT LEE YC
Citation
Lx. Wang et al., A FLUOROMETRIC ASSAY OF CERAMIDE GLYCANASE WITH 4-METHYLUMBELLIFERYL BETA-D-LACTOSIDE DERIVATIVES, Glycoconjugate journal, 13(3), 1996, pp. 359-365
Citations number
18
Categorie Soggetti
Biology
Journal title
Glycoconjugate journal → ACNP
ISSN journal
02820080
Volume
13
Issue
3
Year of publication
1996
Pages
359 - 365
Database
ISI
SICI code
0282-0080(1996)13:3<359:AFAOCG>2.0.ZU;2-T
Abstract
4-Methylumbelliferyl 6'-O-benzyl-beta-D-lactoside (6'Bn-MU-Lac) and so me related compounds were synthesized via different selective reaction s including phase-transfer glycosylation. Their suitability as substra tes for a fluorometric assay of ceramide glycanase (CGase) was evaluat ed. Among others, the 6'Bn-MU-Lac, which is resistant to exo-galactosi dase, was found to be a suitable substrate for routine assay of the CG ase activity. For American leech CGase, the K-m value is 0.232 mM at p H 5.