IN-VITRO INDUCTION OF CYP1A1-ASSOCIATED ACTIVITIES IN HUMAN AND RODENT CELL-LINES BY COMMERCIAL AND TISSUE-EXTRACTED HALOGENATED AROMATIC-HYDROCARBONS

Citation
A. Vamvakas et al., IN-VITRO INDUCTION OF CYP1A1-ASSOCIATED ACTIVITIES IN HUMAN AND RODENT CELL-LINES BY COMMERCIAL AND TISSUE-EXTRACTED HALOGENATED AROMATIC-HYDROCARBONS, Environmental toxicology and chemistry, 15(6), 1996, pp. 814-823
Citations number
41
Categorie Soggetti
Toxicology,"Environmental Sciences",Chemistry
ISSN journal
07307268
Volume
15
Issue
6
Year of publication
1996
Pages
814 - 823
Database
ISI
SICI code
0730-7268(1996)15:6<814:IIOCAI>2.0.ZU;2-8
Abstract
Despite reports of species-specific differences, the rat cell line H4I IE continues to be used to assess the toxicity of many halogenated aro matic hydrocarbon (HAH) contaminants for diverse populations using an approach that relates aryl hydrocarbon (Ah) receptor-mediated response s by HAHs to that of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). To as sess the relevance of H4IIE for human populations, we examined levels of aryl hydrocarbon hydroxylase (AHH) and ethyoxyresorufin O-deethylas e (EROD) activities in H4IIE rat liver, HepG2 human liver, and MCF-7 h uman breast cells treated with TCDD and three coplanar polychlorinated biphenyl (PCB) congeners (77, 126, 169), alone or in combination, and to fractions extracted from the muscle tissue of Lake Ontario rainbow trout (Oncorhynchus ml kiss). H4IIB was generally more sensitive to a ll HAH treatments. Both AHH and EROD activities were induced in all ce lls treated with TCDD and PCB-126, alone or combined with each other. In contrast, neither activity was induced in human cells treated with PCB-77 or PCB-169. For inducing concentrations of PCBs, the induction response in all cell lines treated with 5 nM TCDD-PCB mixtures was gen erally nonadditive; only the response in H4IIE treated with 10(-3) nM TCDD-PCB mixtures was generally additive. With tissue extracts, induct ion was restricted to H4IIE cells treated with TCDD/PCB-or organochlor ide pesticide-containing fractions. Our initial results suggest that d ifferences in the properties of each HAH or in levels of Ah receptor i n each cell line are not likely to underlie the significant difference s in the murine/human induction responses.