IMAGING BY FLUORESCENCE VIDEOMICROSCOPY OF INDIVIDUAL SINGLE-STRANDED-DNA MOLECULES IN SOLUTION

Citation
I. Auzanneau et al., IMAGING BY FLUORESCENCE VIDEOMICROSCOPY OF INDIVIDUAL SINGLE-STRANDED-DNA MOLECULES IN SOLUTION, Comptes rendus de l'Academie des sciences. Serie 3, Sciences de la vie, 316(5), 1993, pp. 459-462
Citations number
7
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
07644469
Volume
316
Issue
5
Year of publication
1993
Pages
459 - 462
Database
ISI
SICI code
0764-4469(1993)316:5<459:IBFVOI>2.0.ZU;2-Z
Abstract
We demonstrate that the observation by fluorescence video-microscopy o f individual single stranded DNA molecules is now possible owing to th e use of an asymmetric cyanine (YOYO-1) belonging to a new class of fl uorescent dyes complexing the DNA. The fluorescence of labelled DNA pe rsists in denaturing conditions showing that the dye does not attach s imply to the possible secondary structures. Furthermore it is stable u nder electrophoresis and enables the imaging of stretched molecules.