Mg. Lambrechts et al., A MULTICOPY SUPPRESSOR GENE, MSS10, RESTORES STA2 EXPRESSION IN SACCHAROMYCES-CEREVISIAE STRAINS CONTAINING THE STA10 REPRESSOR GENE, Current genetics, 29(6), 1996, pp. 523-529
Transcription of the three unlinked, homologous STA1-3 glucoamylase-en
coding genes, involved in starch degradation by Saccharomyces cerevisi
ae, was previously shown to be down-regulated by the presence of STA10
, acting via three upstream repression sequence regions that were iden
tified in the STAZ promoter. Here we report the cloning and characteri
zation of a putative transcriptional activator gene, MSS10 (multicopy
suppressor of STA10), which, when present in multiple copies, overcome
s STA10 repression. Deletion of MSS10, located on chromosome XV, resul
ted in media-specific extinction of glucoamylase synthesis. The nucleo
tide sequence of MSS10 is identical to three other genes from S. cerev
isiae identified as: FUP1, a gene that enhances iron-limited growth; P
HD2, a gene identified for its ability to induce pseudohyphal growth i
n diploid cells grown on nitrogen-limited media; and MSN1, a gene enco
ding a transcriptional activator involved in invertase regulation.