S. Feng et Ptk. Woo, BIOLOGICAL CHARACTERIZATION OF A MONOCLONAL-ANTIBODY AGAINST A SURFACE-MEMBRANE ANTIGEN ON CRYPTOBIA-SALMOSITICA KATZ, 1951, Journal of fish diseases, 19(2), 1996, pp. 137-143
A monoclonal antibody (IgG 1) (designated as MAb-001) was produced aga
inst the pathogenic haemoflagellate Cryptobia salmositica Katz. The an
tibody agglutinated live parasites under in vitro conditions. Live C.
salmositica, incubated with MAb-001 at 10 degrees C, did not multiply
and were dead within 4 weeks in culture. About 50% of juvenile rainbow
trout, Oncorhynchus mykiss (Walbaum), inoculated intraperitoneally wi
th C. salmositica, incubated in MAb-001 prior to inoculation, did not
become infected, while in adult rainbow trout, the peak parasitaemia w
as reduced. These results indicate that MAb-001 is a protective monocl
onal antibody and the antigen it recognizes is located on the surface
membrane of C. salmositica. The antibody also inhibits multiplication
and affects viability of the parasite under in vitro conditions.