USE OF PH STUDIES TO DETERMINE THE KINETIC AND CHEMICAL MECHANISM OF YEAST ALCOHOL-DEHYDROGENASE WITH PRIMARY ALIPHATIC-ALCOHOLS AND ALDEHYDES

Authors
LESKOVAC V TRIVIC S ANDERSON BM
Citation
V. Leskovac et al., USE OF PH STUDIES TO DETERMINE THE KINETIC AND CHEMICAL MECHANISM OF YEAST ALCOHOL-DEHYDROGENASE WITH PRIMARY ALIPHATIC-ALCOHOLS AND ALDEHYDES, Indian Journal of Biochemistry & Biophysics, 33(3), 1996, pp. 177-183
Citations number
26
Categorie Soggetti
Biophysics,Biology
Journal title
Indian Journal of Biochemistry & Biophysics → ACNP
ISSN journal
03011208
Volume
33
Issue
3
Year of publication
1996
Pages
177 - 183
Database
ISI
SICI code
0301-1208(1996)33:3<177:UOPSTD>2.0.ZU;2-W
Abstract
A complete list of all steady-state:kinetic constants for the yeast al cohol dehydrogenase (EC 1.1.1.1) catalyzed oxidation of ethanol, propa n-1-ol and butan-1-ol, and for the reduction of acetaldehyde and propi onaldehyde was collected in the pH range 6-10, and an appropriate pH p rofile for each constant was constructed, A common minimal mechanism w ith all these substrates has been postulated and pK(a) values and the pH independent limiting values have been assigned for the rate constan ts.