INFRARED-SPECTRA OF URINE FROM CANCEROUS BLADDERS

The infrared spectra of organic constituents of urine from cancerous b ladders of some patients were recorded. The spectra of the organic par t of the samples were classified into five types according to the bulk constituents. Samples with type A spectra consisted mainly of protein s with only trace amounts of lipids. Their spectra were characterized mainly by the absorption bands of proteins at the frequencies 3330, 30 75, 2960, 2850, 1650, 1530, 1450, 1400 and 1320 cm(-1), in addition to a weak band at 1720 cm(-1) due to the absorption of lipids. Samples w ith type B spectra were characterized by high amounts of proteins and low amounts of lipids and phosphate compounds. The presence of phospha te compounds was indicated by the absorption bands at the frequencies 1100 and 1030 cm(-1). Samples giving spectral type C were characterize d by high urea contents as indicated by the presence of two strong ban ds at 1670 and 1630 cm(-1). Samples with the spectral type D consisted of urea and phosphate compounds whereas the last spectral type E cons isted mainly of calcium oxalates, uric acids and phosphate compounds. The presence of calcium oxalates was indicated by the presence of its diagnostic bands at the frequencies 1630 and 1330 cm(-1), while the pr esence of uric acid was indicated by the bands at the frequencies 1360 , 1130, 1020 and 880 cm(-1). On the other hand, the spectra of the org anic part of urine from some normal bladders exhibited the characteris tic bands of urea only. Careful examination of the spectra of the inor ganic part of urine revealed that same samples consisted mainly of hyd roxyapatite. The absorption bands of hydroxyapatite appeared at the fr equencies 568, 603, 985, 1037 and 1128 cm(-1). The spectra of other sa mples showed that the bands of basic phosphates at the frequencies 568 , 620, 727, 890, 1035 and 1140 cm(-1). The spectra of the inorganic pa rt of urine from a number of normal bladders displayed the bands of ba sic phosphates. The relationship between urine constituents and pathol ogical types of bladder tumor tissue was discussed.