MATHEMATICAL-MODELING AND OPTIMIZATION OF PLASMID-HARBORING AND CHROMOSOME-INTEGRATED RECOMBINANT YEAST CULTURE PROCESSES

For the enhanced secretion of foreign proteins, the mathematical model ing for overall recombinant yeast culture considering protein secretio n dynamics is important because we can understand and predict the beha vior of biosynthesis and secretion of foreign protein and can develop control strategies for feeding with these model equations. In this res earch, the mathematical modeling and simulation considering protein se cretion dynamics for recombinant yeast cultures that contain multicopy plasmids or chromosomally integrated genes were performed for optimiz ation of foreign glucoamylase production. The optimal feeding policy f or maximizing glucoamylase production was suggested in fed-batch cultu re. By introducing this optimal feeding policy, final glucoamylase act ivity and productivity of fed-batch culture were significantly increas ed compared with those of batch culture in both recombinant yeasts.