MOUSE G2-GPI ACHE IS PROCESSED AS A MEMBRANE-BOUND ECTOENZYME IN TRANSFECTED MOUSE SARCOMA-CELLS BUT IS NOT A HEMOPHILIC ADHESION MOLECULE

Acetylcholinesterase (AChE) is mainly involved in synaptic transmissio n by hydrolyzing acetylcholine in the synaptic cleft. It has been sugg ested that it could also be involved in other functions such as cell-c ell adhesion. In this study, we have expressed mouse G2-GPI AChE at th e membrane surface of S180 cells. We obtained a transfected cell line which permanently expresses high levels of AChE at the cell surface. H owever, transfected cells behave as single cells in culture. We perfor med cell aggregation and adhesion tests and found no significant aggre gation or adhesion, which suggests that AChE is not a hemophilic adhes ion molecule.