THE POLYETHER BISTRATENE-A ACTIVATES PROTEIN-KINASE-C-DELTA AND INDUCES GROWTH ARREST IN HL-60 CELLS

Bistratene A (BisA) induced growth arrest in G2/M in HL60 cells. In ad dition, BisA-treated cells (50nM for 48 h) became adherent and express ed the adhesion molecule CD11c, but did not express the monocyte enzym e alpha-napthyl acetate esterase or phagocytose complement coated yeas ts. BisA activated protein kinase C (PKC)-delta and induced translocat ion of PKC-delta to the nucleus. This suggests that activation of PKC- delta can induce growth arrest and cell adhesion, but is insufficient to mediate full differentiation of HL60 cells. BisA has potential asa new probe for determining the function of PKC isoenzymes, specifically PKC-delta. (C) 1996 Academic Press, Inc.