A PARA-SITE SPECIFIC HYDROXYLATION OF VARIOUS AROMATIC-COMPOUNDS BY MYCOBACTERIUM SP STRAIN 12523

Authors
SEMBA H MUKOUYAMA M SAKANO K
Citation
H. Semba et al., A PARA-SITE SPECIFIC HYDROXYLATION OF VARIOUS AROMATIC-COMPOUNDS BY MYCOBACTERIUM SP STRAIN 12523, Applied microbiology and biotechnology, 46(4), 1996, pp. 432-437
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Applied microbiology and biotechnology → ACNP
ISSN journal
01757598
Volume
46
Issue
4
Year of publication
1996
Pages
432 - 437
Database
ISI
SICI code
0175-7598(1996)46:4<432:APSHOV>2.0.ZU;2-D
Abstract
A screening of microorganisms with para-site-specific hydroxylation ac tivity of aromatic compounds has been carried out by a three-step scre ening procedure involving a newly established micro-plate assay method . About 1300 strains isolated from about 5000 soils showed the activit y, The hydroxylation of aromatic compounds by one of the isolates, des ignated Mycobacterium sp. strain 12523, which had the highest activity , was examined in detail. It produced p-phenols with high selectivity without o- or m-site hydroxylation. The yield of hydroquinone from phe nol by strain 12523 was 97 mol%.