A PORCINE MODEL OF CHRONIC PERIPHERAL ARTERIAL-OCCLUSION

PURPOSE: To create a simple and reproducible model of chronic thrombos is for the evaluation of thrombolytic agents and devices. MATERIALS AN D METHODS: A stenosis was created in the superficial femoral artery of domestic swine, and autologous blood clot was deposited above the ste nosis. Follow-up lasted for up to 3 months. Degree of clot organizatio n was determined at histologic examination. Two thrombolytic agents, u rokinase and collagenase, were used to test this model. RESULTS: There was a 27% delayed recanalization rate with this model. At histologic examination, early thrombus organization was seen at the vessel periph ery after 10 days. One-month-old thrombus was substantial but variable in amount. Three-month-old thrombus was completely organized. Neither urokinase nor collagenase proved effective against chronic clot in th e doses and time course of this study. CONCLUSION: This simple method yields a chronic porcine clot in a reliable number of domestic swine i n 1 month.