AN APPROACH TO MODIFIED HETEROCYCLIC-ANALOGS OF HUPERZINE-A AND ISOHUPERZINE-A - SYNTHESIS OF THE PYRIMIDONE AND PYRAZOLE ANALOGS, AND THEIR ANTICHOLINESTERASE ACTIVITY
Ap. Kozikowski et al., AN APPROACH TO MODIFIED HETEROCYCLIC-ANALOGS OF HUPERZINE-A AND ISOHUPERZINE-A - SYNTHESIS OF THE PYRIMIDONE AND PYRAZOLE ANALOGS, AND THEIR ANTICHOLINESTERASE ACTIVITY, Journal of the Chemical Society. Perkin transactions. I, (11), 1996, pp. 1287-1297
Synthetic approaches to the pyrimidone and the pyrazole analogues of t
he naturally occurring acetylcholinesterase (AChE) inhibitor huperzine
A and its unnatural regioisomer, isohuperzine, are described, The pyr
imidone analogues of huperzine A were obtained starting from cyclohexa
ne-1,4-dione monoethylene ketal by first annealing to this monocycle a
pyrimidine ring and then constructing the unsaturated three-carbon br
idge using the previously described palladium-catalysed bicycloannulat
ion methodology, A major problem in this synthetic undertaking proved
to be introduction of the ethylidene appendage onto tricycle 10, While
both Wittig and Takai olefination protocols proved unsuccessful, the
ethylidene moiety was eventually introduced using the Danheiser method
ology which involves a two step reaction sequence consisting of the in
termediate construction of a beta-lactone, which in turn undergoes a [
2 + 2] cycloreversion leading to the desired olefin. This beta-lactone
synthesis, which has not-previously been applied to beta-keto esters,
was found to proceed with excellent diastereoselectivity. In turn, th
e beta-lactone underwent a stereospecific decarboxylation reaction to
provide the E-olefin product as the sole isomer. Additionally, startin
g from the bicycle[3.3.1] nonene intermediate 2 we describe a syntheti
c strategy for procuring modified heterocyclic analogues of isohuperzi
ne A. This chemistry provides an attractive approach to the synthesis
of heterocyclic analogues with unsaturation in the 6,7 position. While
none of these new analogues was found to rival huperzine A in its abi
lity to act as a reversible inhibitor of AChE, the data reported herei
n should prove useful to modeling efforts aimed at acquiring a better
understanding of huperzine A's binding topography within AChE.