SEROLOGICALLY DEFINED GENOTYPES OF HEPATITIS-C VIRUS AMONG JAPANESE PATIENTS WITH CHRONIC HEPATITIS-C

Recently, Tsukiyama-Kohara et al. (1993) reported that most hepatitis C viruses (HCVs) in Japan can be classified into two types, type 1 and type 2, on the basis of the NS4 region nucleotide sequence. They deve loped a new assay in which antibodies against group-specific recombina nt proteins of the NS4 region were measured by ELISA (serologically de fined genotype, serotype). In the present study, we examined 306 patie nts with chronic liver disease due to HCV infection. The sensitivity o f this assay was 98.7% (302/306), The serotype distribution of HCV was 239/305 (75.2%) for type 1, 65/306 (21.2%) for type 2, 7/306 (2.3%) f or mixed, both being positive, and 4/306 (1.3%) indeterminate. The fre quency of type 1 was significantly higher than that of type 2 (P < 0.0 1). There were no significant differences in clinical characteristics among the mixed and the indeterminate serotypes. Among the mixed-serot ype patients, 4/7 (57.1%) showed seroconversion to a single serotype a t 6 and 9 months later, although the serotypes of the indeterminate-se rotype patients were also indeterminate at 6 and 9 months later. Using aliquots of the same serum samples: HCV genotyping was carried out by the reverse transcription polymerase chain reaction (RT-PCR) method u sing type-specific primers derived from the NS5 region of HCV to verif y the specificity of this serotyping. The sensitivity of genotyping by RT-PCR was 167/183 (91.3%). The HCV genotypes determined by both meth ods were consistent in 161/183 (88.0%) of the cases, and there were no contradictory results for any sample between the two methods. These f indings indicate that serological genotyping might be useful in determ ining the HCV genotype among Japanese patients with HCV infection.