Bj. Collins et al., PRESENCE OF RABBIT HEMORRHAGIC-DISEASE VIRUS-ANTIGEN IN RABBIT-TISSUES AS REVEALED BY A MONOCLONAL-ANTIBODY DEPENDENT CAPTURE ELISA, Journal of virological methods, 58(1-2), 1996, pp. 145-154
Citations number
30
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
The production of monoclonal antibodies (mAbs) to a common antigenic r
egion on rabbit haemorrhagic disease virus (RHDV) has enabled the deve
lopment of a capture ELISA for virus detection. The assay was shown to
detect reliably the presence of viral antigen in crude homogenates of
a range of rabbit tissues and has provided the first evidence for the
presence of RHDV in blood, serum and heart tissue. A limited time cou
rse study of the progression of virus infection revealed viral antigen
is first detected in the liver at between 18 and 24 h post-infection
(p.i.) and in the spleen between 24 and 30 h p.i. The assay displayed
a high level of specificity, clearly differentiating between groups of
infected (lowest observed optical density (O.D.) 0.45) and uninfected
(highest O.D. 0.06) rabbits. No viral antigen was detected in the uri
ne or faeces collected from infected rabbits at post-mortem. Experimen
ts employing the 'spiking' of pools of urine and faecal homogenates co
llected from uninfected rabbits, with a known amount of virus, indicat
ed that RHDV may not be present in the faeces of infected animals and
if present in urine, it appears to undergo substantial degradation.