CHLORHEXIDINE CHLOROCRESOL ASSOCIATION - INVITRO STUDY OF INTERACTIONS AGAINST POTENTIAL CUTANEOUS STRAINS

The interaction type of chlorhexidine digluconate/chlorocresol associa tion (foaming solution DC 102/1, Laboratoires Pierre Fabre Medicament) have been studied according to the chess-board method, on microplate, by determining MBC (Minimal Bactericidal Concentrations) (or MFC (Min imal Fungicidal Concentrations)) adjusted to T 72-150 AFNOR norm (dilu tion-neutralization, conlact 5 mn) and computation of FBC (or FFC) Ind ex. Trials were carried out against 18 hospital strains (Staphylococcu s epidermidis, Staphylococcus aureus, Propionibacterium acnes, Candida albicans) and against 4 strains of the AFNOR norm. MBC of chlorhexidi ne, againts Staphylococci were included between 6.25 and 25 mug/ml, an d between 25 and 200 mug/ml against the others strains. MBC of chloroc resol were included between 800 and 3 200 mug/ml. Against the 22 teste d strains, 18 synergistic interactions (FBC Index less-than-or-equal-t o 0,75) were observed, with 4 to 8 reducing factor for chlorhexidine, and greater-than-or-equal-to 2 for chlorocresol. No antagonism was not ed (maximal FBC Index = 1).