THE USE OF AN AUTOMATED-ASSAY TO ASSESS PHAGE SURVIVAL AFTER A BIOCIDAL TREATMENT

Authors
MAILLARD JY BEGGS TS DAY MJ HUDSON RA RUSSELL AD
Citation
Jy. Maillard et al., THE USE OF AN AUTOMATED-ASSAY TO ASSESS PHAGE SURVIVAL AFTER A BIOCIDAL TREATMENT, Journal of Applied Bacteriology, 80(6), 1996, pp. 605-610
Citations number
13
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Journal of Applied Bacteriology → ACNP
ISSN journal
00218847
Volume
80
Issue
6
Year of publication
1996
Pages
605 - 610
Database
ISI
SICI code
0021-8847(1996)80:6<605:TUOAAT>2.0.ZU;2-D
Abstract
The coliphage K-1-5 has been used in an automated assay to monitor the viricidal activity of various disinfectants. This indirect assay base d on the spectrophotometric reading of the lysis of the host cell (Esc herichia coli D837) produced encouraging results and was faster than t he overlay counting method (previously studied) which relies on plaque formation. However, differences in sensitivity towards some disinfect ants were observed between the two methods.