ULTRAFILTRATION DISCREPANCIES IN RECOVERY OF MYOGLOBIN FROM URINE

Citation
B. Loun et al., ULTRAFILTRATION DISCREPANCIES IN RECOVERY OF MYOGLOBIN FROM URINE, Clinical chemistry, 42(6), 1996, pp. 965-969
Citations number
24
Categorie Soggetti
Chemistry Medicinal
Journal title
ISSN journal
00099147
Volume
42
Issue
6
Year of publication
1996
Part
1
Pages
965 - 969
Database
ISI
SICI code
0009-9147(1996)42:6<965:UDIROM>2.0.ZU;2-X
Abstract
We investigated the efficiency, accuracy, and reliability of the ultra filtration/dipstick methodology commonly used to diagnose myoglobinuri a. Twenty-five myoglobin-containing urine specimens were filtered by c entrifugation for 15 min at 1500g through a Centricon-30(TM) membrane filter, Both the original specimen and filtrate were assayed for myogl obin. The amount of myoglobin recovered subsequent to filtration varie d from <1-38%, This poor and variable recovery was independent of samp le matrix or precentrifugation of the specimens, This was most critica l for urine specimens with myoglobin concentrations <60 000 mu g/L. Fo urteen of 18 such filtrates had concentrations <350 mu g/L, a concentr ation below which a negative result would be obtained by using convent ional dipstick methods, Thus, the use of this procedure has the potent ial to misdiagnose patients with myoglobin concentrations associated w ith increased risk of subsequent renal dysfunction, in particular when urine myoglobin concentrations are <60 000 mu g/L.