E. Armstrong et al., FLT4 RECEPTOR TYROSINE KINASE GENE-MAPPING TO CHROMOSOME BAND 5Q35 INRELATION TO THE T(2 5), T(5 6), AND T(3 5) TRANSLOCATIONS, Genes, chromosomes & cancer, 7(3), 1993, pp. 144-151
FLT4 is a recently cloned receptor tyrosine kinase cDNA, which is char
acterized by seven immunoglobulin-like loops in its extracellular doma
in. We have previously mapped the FLT4 gene to chromosome segment 5q33
-qter using somatic cell hybrids. Here we have refined the localizatio
n to band 5q35 by fluorescence in situ hybridization and show that the
gene is translocated to chromosomes 2 and 6 in the t(2;5)(p23;q35) an
d t(5;6)(q35;p21) translocations, respectively, of Ki-1-positive lymph
omas, as well as to chromosome 3 in the t(3;5)(q25.1;q34) translocatio
n, which is occasionally found in myelodysplastic syndromes and acute
myeloid leukemia. No evidence was obtained for a rearrangement or dere
gulation of the translocated FLT4 gene. We further show that abundant
FLT4 mRNA expression occurs only in erythroid and megakaryoblastoid ce
ll lines among nine leukemia cell lines studied. (C) 1993 Wiley-Liss,
Inc.