CLONAL CYTOGENETIC EVOLUTION IN A SQUAMOUS-CELL CARCINOMA OF THE SKINFROM A XERODERMA-PIGMENTOSUM PATIENT

Cytogenetic changes in epithelial neoplasms are often complex, making it difficult to determine which are the primary abnormalities and whic h are secondary. In this report, we describe clonal evolution in a cyt ogenetically simple, but clinically aggressive squamous cell cancer of the skin from a patient with xeroderma pigmentosum. Chromosome prepar ations were analyzed from a direct harvest, three independent primary harvests, and passaged cell lines. Three closely related tumor subclon es were identified in the primary cultures, and all three proliferated in vitro. Monosomy 4 and a chromosome 9 rearrangement were present in all three subclones and monosomy 21 was present in two subclones. An i(9p) and an i(9q) were derived from the same chromosome 9, and there was concurrent loss of the homologous chromosome 9. In addition, each subclone was characterized by a further specific evolutionary change: t(5;7) (q11.2;p22) in subclone 1, der (11)t(10;11) (q21;p14) in subclo ne 2, and der (14)t(13;14) (q14;q32) in subclone 3. All three subclone s were represented by hypodiploid and hypotetraploid metaphases. Loss of chromosomes from hypotetraploid cells and an 11q+ were the only oth er changes found in this tumor. The early genetic events in the evolut ion of this squamous cell cancer, monosomy 4, i(9p), i(9q), and monoso my 21, represent loss of chromosome regions that are commonly lost in other squamous cell carcinomas of the head and neck region. Taken toge ther, these observations indicate that genes on these chromosomal regi ons are probably important and possibly sufficient for the development of squamous cell carcinoma. (C) 1993 Wiley-Liss, Inc.