POLYLYSINE ENHANCES CATIONIC LIPOSOME-MEDIATED TRANSFECTION OF THE HEPATOBLASTOMA CELL-LINE HEP G2

Plasmid DNA condensed by polylysine enhanced cationic-liposome-mediate d transfection of Hep G2 cells, The luciferase expression plasmid pCMV L was complexed with the polycation poly-L-lysine and mixed with lipos omes that contained a I:I molar ratio of the cationic lipid 1,2-dioleo yloxy-3-trimethylammoniumpropane, with the neutral phospholipid -9-oct adecenoyl)-sn-glycero-3-phosphoethanolamine. Polylysine enhanced catio nic-liposome-mediated transfection of the hepatoblastoma cell line Hep GZ 9-fold compared with pCMVL complexed alone with liposomes, The rat io of cationic to anionic charge of the polylysine-pCMVL complexes, an d the quantity of cationic liposomes, are important determinants for o ptimal transfection of Hep G2 cells.