PROTEIN-KINASE C-ALPHA,C-BETA IMMUNOREACTIVITY IN BAKERS-YEAST, LOBSTER AND WHEAT-GERM

The immunoreactivity of PKCalpha(protein kinase C-alpha) and PKCbeta i n wheat germ, lobster tail muscle and three strains of yeast was analy sed by Western blotting with mouse anti-PKC active fragments. The pote ncy of the immunoreactivity of PKCalpha activity was much greater than that of PKCbeta. The occurrence of multiple bands may be due to PKC s elf-interactions and/or the interactions between PKC and other molecul es. The evolutionary conservation of PKCalpha and PKCbeta implies that these PKC isoenzymes may play important roles in Ca2+/lipid-dependent signal transduction and cell growth in these eukaryotes.