CONSTRUCTION OF A YAC CONTIG ENCOMPASSING THE USHER SYNDROME TYPE 1C AND FAMILIAL HYPERINSULINISM LOCI ON CHROMOSOME 11P14-15.1

The Usher syndrome type 1C (USH1C) and familial hyperinsulinism (HI) l oci have been assigned to chromosome 11p14-15.1, within the interval D 11S419-D1151310. We have constructed a yeast artificial chromosome (YA C) contig, extending from D11S926 to D11S899, which encompasses the cr itical regions for both USH1C and HI and spans an estimated genetic di stance of approximate to 4 cM. A minimal set of six YAC clones constit ute the contig, with another 22 YACs confirming the order of sequence- tagged sites (STSs) and position of YACs on the contig. A total of 40 STSs, including 10 new STSs generated from YAC insert-end sequences an d inter-Alu PCR products, were used to order the clones within the con tig. This physical map provides a resource for identification of gene transcripts associated with USH1C, HI, and otter genetic disorders tha t map to the D11S926-D11S899 interval.