EXTRACTION OF SALINOMYCIN FROM FINISHED LAYERS RATION BY MICROWAVE SOLVENT-EXTRACTION FOLLOWED BY LIQUID-CHROMATOGRAPHY

A rapid, reliable, environmentally friendly and economical extraction technique was developed for the extraction of sodium salinomycin (refe rred to as salinomycin antibiotic to prevent coccidiosis in broilers) in finished feeds, The method involved a microwave extraction procedur e using an ordinary microwave oven, Feed mixed with ethanol-propan-2-o l (15 + 2) was irradiated with microwave energy for 8 s, Salinomycin w as detected by HPLC at 592 nm after post-column derivatization with di methylaminobenzaldehyde at 85 degrees C, The salinomycin concentration determined in finished feeds was 34, 60, 103 and 160 mg kg(-1), compa red with calculated values of 30, 60, 90 and 150 mg kg(-1), respective ly, The corresponding values obtained with the conventional technique were 27, 45, 59 and 112 mg kg(-1). Salinomycin is stable in finished f eeds for more than 8 months.